Re: [AMBER] nmr refinment problem

From: case <>
Date: Tue, 15 Jun 2010 09:21:12 -0400

On Tue, Jun 15, 2010, shirishkumar pawar wrote:

> i am first time amber user. i want to do nmr refinment of homology model of
> one of the gpcr receptor. what all requirements are there for this work. now
> i am following *AMBER ADVANCED WORKSHOP** TUTORIAL 4* for *AMBER Refinement
> of a DNA Duplex*. will it be different in case of protein? can you suggest
> any good tutorial for nmr refinment of a protein.

The principles of NMR refinement are the same for proteins as for nucleic

> also as my protein is
> gpcr, the no of amino acids are upto 370, so, how many days/months it will
> take to complete this process?

The actual calculations typically take a few hours per structure, and people
often refine 100 structures or so, then choose the best dozen or two. But
learning to create the input and intelligently run the jobs may be time
consuming for a newcomer.

...good luck...dac

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Received on Tue Jun 15 2010 - 06:30:07 PDT
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