G5 C A A A A T T T T G C3
C5 G T T T T A A A A C G3
I think nucgen reads and generates 5'->3', where in nature
the 5' base is opposite the 3' base of the other strand.
So your base *pairs* defined above are:
G5 C A A A A T T T T G C3
: : :
G3 C A ................................ C5
Therefore a complementary 2nd strand in nuc.in would begin
G5 C A A ...
Bill
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Received on Tue Jun 07 2005 - 20:53:00 PDT
