Hi Kenneth,
exactly! I've stripped solvent first using cpptraj from the 3
trajectories merged together within same script and than proceed both
stripped topologies and trajectory (only protein and ligand) to the
ante-mmpbsa to make 3 separate prmtop from which complex.prmtop was
equal to the stripped.prmtop which I've used as the input. In that
case no errors has been occurred during mmgbsa.py processing.
HTH,
James
2015-04-06 17:25 GMT+02:00 Kenneth Huang <kennethneltharion.gmail.com>:
> That's great to hear!
>
> Though, I am a little confused as to what you mean about using the stripped
> topologies/trajectories for ante-MMPBSA- did you mean that you stripped the
> trajectory and topology with cpptraj, and then used the stripped topology
> in ante-MMPBSA as the complex? Just wondering, both for future reference if
> anyone else runs into the problem and to see what exactly is going wrong in
> the topology creation step.
>
> Best,
>
> Kenneth
>
> On Monday, April 6, 2015, James Starlight <jmsstarlight.gmail.com> wrote:
>
>> solved solved ! )
>> What I've done is
>>
>> 1) providing protein.inpcrd at the reference during cutting of my
>> trajectories using above script.
>>
>> 2) stripping solvent manually using cpptraj providing to ante-mmpbsa
>> stripped trajectories and topologies as the input to made complex
>> (which will be the same as the input topology in this case) as well as
>> for complex and ligand.
>>
>> After this mmbsa have been finished without any error.
>>
>> In any of case thank for the help!
>>
>> Regards,
>>
>> James
>>
>> 2015-04-06 16:23 GMT+02:00 Kenneth Huang <kennethneltharion.gmail.com
>> <javascript:;>>:
>> > Assuming that you've treated the trajectories all the same before or
>> after
>> > merging them, ie applying the same strip masks, etc, then there shouldn't
>> > be a problem.
>> >
>> > I don't think MMPBSA should care too much about where your coordinates
>> are,
>> > though it never hurts to autoimage and center your trajectory to a
>> > reference. This seems more to be there's something present in the
>> > trajectory or topology from the error message.
>> >
>> > Best,
>> >
>> > Kenneth
>> >
>> > On Monday, April 6, 2015, James Starlight <jmsstarlight.gmail.com
>> <javascript:;>> wrote:
>> >
>> >> one important suggestion regarding my issue:
>> >>
>> >> I've tried to make this type of mmgbsa for merged trajectory consisted
>> >> of 3 trajectory (each of which has been calculated for same input
>> >> prmtop file) using below script to cut it together:
>> >>
>> >> parm protein.parm7
>> >> trajin md1.nc 100 last 1
>> >> trajin md2.nc 100 last 1
>> >> trajin md3.nc 100 last 1
>> >> trajout merged_md1_2_3.nc netcdf
>> >>
>> >> should I provide here some reference also to superimpose all of those
>> >> trajectories onto common reference? What also might be relevant?
>> >>
>> >> James
>> >> ~
>> >> ~
>> >> ~
>> >> ~
>> >> ~
>> >>
>> >> 2015-04-06 15:12 GMT+02:00 James Starlight <jmsstarlight.gmail.com
>> <javascript:;>
>> >> <javascript:;>>:
>> >> > no no it was exactly paramtop of the whole system loaded with full
>> >> > trajectory as you can found from
>> >> >
>> >> > 0: OR5P3_plus-carvone.parm7, 44513 atoms, 9262 res, box: Orthogonal,
>> >> > 8970 mol, 8856 solvent, 5901 frames
>> >> > just try to switch to the ambertools-14 and let you know about success
>> >> >
>> >> >
>> >> >
>> >> > James
>> >> >
>> >> > 2015-04-03 17:22 GMT+02:00 Kenneth Huang <kennethneltharion.gmail.com
>> <javascript:;>
>> >> <javascript:;>>:
>> >> >>>
>> >> >>> how it would be possible to obtain some coordinates just from the
>> >> >>> topology and trajectory using ambpdb? I'm working on cluster where
>> >> >>> unfortunately there is no any visualization software.
>> >> >>
>> >> >>
>> >> >> The general command for ambpdb is
>> >> >>
>> >> >> ambpdb -p topology.prmtop < restart.inpcrd > OutputName.pdb
>> >> >>
>> >> >> Usually I use it with the starting .inpcrd or .restrt files- I
>> haven't
>> >> >> tried it with a trajectory, but I assume that you can just save a
>> single
>> >> >> frame, and use that as your coordinate file for ambpdb.
>> Alternatively,
>> >> you
>> >> >> can save a single frame and move it along with the topologies onto a
>> >> local
>> >> >> machine to try to visualize it there.
>> >> >>
>> >> >>
>> >> >>> also I've load both prmtop and nc into the cpptraj and no mismatch
>> has
>> >> >>> been found
>> >> >>
>> >> >>
>> >> >> I could be wrong, but it looks like you've selected the initial
>> prmtop
>> >> and
>> >> >> your trajectory- try loading a stripped version of your trajectory
>> >> (using
>> >> >> the same masks that you chose in running ante-MMPBSA) and its
>> >> corresponding
>> >> >> complex prmtop. Since I assume you can load your initial
>> >> prmtop/trajectory
>> >> >> without issue, I'm thinking that the issue is somewhere in the
>> >> >> complex.prmtop file.
>> >> >>
>> >> >> just noticed that on cluster I'm using amber-tools-13. Was some bug
>> >> >>> within mmpbsa.py of amber-tools of this version?
>> >> >>
>> >> >>
>> >> >> I'm not sure if AmberTools13 had any problems associated with this,
>> so
>> >> >> you'd have to wait for someone else to weigh in. That said, I'm
>> usually
>> >> in
>> >> >> favor of using newer versions of AmberTools, ie AmberTools14, unless
>> you
>> >> >> have a specific reason not to.
>> >> >>
>> >> >> Best,
>> >> >>
>> >> >> Kenneth
>> >> >>
>> >> >> On Friday, April 3, 2015, James Starlight <jmsstarlight.gmail.com
>> <javascript:;>
>> >> <javascript:;>> wrote:
>> >> >>
>> >> >>> also I've load both prmtop and nc into the cpptraj and no mismatch
>> has
>> >> >>> been found
>> >> >>>
>> >> >>> CPPTRAJ: Trajectory Analysis. V13.24
>> >> >>> ___ ___ ___ ___
>> >> >>> | \/ | \/ | \/ |
>> >> >>> _|_/\_|_/\_|_/\_|_
>> >> >>> INPUT: Reading Input from file xz.in
>> >> >>> [parm OR5P3_plus-carvone.parm7]
>> >> >>> AmberParm Title: [default_name]
>> >> >>> Radius Set: modified Bondi radii (mbondi)
>> >> >>> [trajin OR5P3_plus-carvone.nc netcdf 100 last 1]
>> >> >>> [OR5P3_plus-carvone.nc] contains 6000 frames.
>> >> >>> [trajout md_5p3_coumarin_md1_2.dcd dcd first]
>> >> >>>
>> >> >>> PARAMETER FILES:
>> >> >>> 0: OR5P3_plus-carvone.parm7, 44513 atoms, 9262 res, box:
>> Orthogonal,
>> >> >>> 8970 mol, 8856 solvent, 5901 frames
>> >> >>>
>> >> >>> INPUT TRAJECTORIES:
>> >> >>> 0: [OR5P3_plus-carvone.nc] is a NetCDF AMBER trajectory, Parm
>> >> >>> OR5P3_plus-carvone.parm7 (Orthogonal box) (reading 5901 of 6000)
>> >> >>> Coordinate processing will occur on 5901 frames.
>> >> >>>
>> >> >>>
>> >> >>> just noticed that on cluster I'm using amber-tools-13. Was some bug
>> >> >>> within mmpbsa.py of amber-tools of this version?
>> >> >>>
>> >> >>> J
>> >> >>>
>> >> >>> 2015-04-03 13:53 GMT+02:00 James Starlight <jmsstarlight.gmail.com
>> <javascript:;>
>> >> <javascript:;>>:
>> >> >>> > I've checked it- solvent has been stripped correctly! and yes I'm
>> >> >>> > using the same prmtop which I've used as the input to produce
>> >> >>> > trajectory
>> >> >>> > residues from complex:
>> >> >>> > %FORMAT(20a4)
>> >> >>> > GLU ASP THR THR VAL CYS ALA ILE LEU PHE LEU VAL PHE LEU GLY ILE
>> TYR
>> >> VAL
>> >> >>> VAL THR
>> >> >>> > LEU MET GLY ASN ILE SER ILE ILE VAL LEU ILE ARG ARG SER HID HIP
>> LEU
>> >> HID
>> >> >>> THR PRO
>> >> >>> > MET TYR ILE PHE LEU CYS HID LEU ALA PHE VAL ASH ILE GLY TYR SER
>> SER
>> >> SER
>> >> >>> VAL THR
>> >> >>> > PRO VAL MET LEU MET SER PHE LEU ARG LYS GLH THR SER LEU PRO VAL
>> ALA
>> >> GLY
>> >> >>> CYX VAL
>> >> >>> > ALA GLN LEU CYS SER VAL VAL THR PHE GLY THR ALA GLH CYS PHE LEU
>> LEU
>> >> ALA
>> >> >>> ALA MET
>> >> >>> > ALA TYR ASP ARG TYR VAL ALA ILE CYS SER PRO LEU LEU TYR SER THR
>> CYS
>> >> MET
>> >> >>> SER PRO
>> >> >>> > GLY VAL CYS ILE ILE LEU VAL GLY MET SER TYR LEU GLY GLY CYS VAL
>> ASN
>> >> ALA
>> >> >>> TRP THR
>> >> >>> > PHE ILE GLY CYS LEU LEU ARG LEU SER PHE CYS GLY PRO ASN LYS VAL
>> ASN
>> >> HIP
>> >> >>> PHE PHE
>> >> >>> > CYX ASP TYR SER PRO LEU LEU LYS LEU ALA CYS SER HID ASP PHE THR
>> PHE
>> >> GLU
>> >> >>> ILE ILE
>> >> >>> > PRO ALA ILE SER SER GLY SER ILE ILE VAL ALA THR VAL CYS VAL ILE
>> ALA
>> >> ILE
>> >> >>> SER TYR
>> >> >>> > ILE TYR ILE LEU ILE THR ILE LEU LYS MET HID SER THR LYS GLY ARG
>> HIE
>> >> LYS
>> >> >>> ALA PHE
>> >> >>> > SER THR CYS THR SER HID LEU THR ALA VAL THR LEU PHE TYR GLY THR
>> ILE
>> >> THR
>> >> >>> PHE ILE
>> >> >>> > TYR VAL MET PRO LYS SER SER TYR SER THR ASP GLN ASN LYS VAL VAL
>> SER
>> >> VAL
>> >> >>> PHE TYR
>> >> >>> > THR VAL VAL ILE PRO MET LEU ASN PRO LEU ILE TYR SER LEU ARG ASN
>> LYS
>> >> GLU
>> >> >>> ILE LYS
>> >> >>> > GLY ALA LEU LYS ARG GLU LEU ARG ILE LYS ILE PHE SER MOL
>> >> >>> >
>> >> >>> > residues from receptor:
>> >> >>> >
>> >> >>> > %FORMAT(20a4)
>> >> >>> > GLU ASP THR THR VAL CYS ALA ILE LEU PHE LEU VAL PHE LEU GLY ILE
>> TYR
>> >> VAL
>> >> >>> VAL THR
>> >> >>> > LEU MET GLY ASN ILE SER ILE ILE VAL LEU ILE ARG ARG SER HID HIP
>> LEU
>> >> HID
>> >> >>> THR PRO
>> >> >>> > MET TYR ILE PHE LEU CYS HID LEU ALA PHE VAL ASH ILE GLY TYR SER
>> SER
>> >> SER
>> >> >>> VAL THR
>> >> >>> > PRO VAL MET LEU MET SER PHE LEU ARG LYS GLH THR SER LEU PRO VAL
>> ALA
>> >> GLY
>> >> >>> CYX VAL
>> >> >>> > ALA GLN LEU CYS SER VAL VAL THR PHE GLY THR ALA GLH CYS PHE LEU
>> LEU
>> >> ALA
>> >> >>> ALA MET
>> >> >>> > ALA TYR ASP ARG TYR VAL ALA ILE CYS SER PRO LEU LEU TYR SER THR
>> CYS
>> >> MET
>> >> >>> SER PRO
>> >> >>> > GLY VAL CYS ILE ILE LEU VAL GLY MET SER TYR LEU GLY GLY CYS VAL
>> ASN
>> >> ALA
>> >> >>> TRP THR
>> >> >>> > PHE ILE GLY CYS LEU LEU ARG LEU SER PHE CYS GLY PRO ASN LYS VAL
>> ASN
>> >> HIP
>> >> >>> PHE PHE
>> >> >>> > CYX ASP TYR SER PRO LEU LEU LYS LEU ALA CYS SER HID ASP PHE THR
>> PHE
>> >> GLU
>> >> >>> ILE ILE
>> >> >>> > PRO ALA ILE SER SER GLY SER ILE ILE VAL ALA THR VAL CYS VAL ILE
>> ALA
>> >> ILE
>> >> >>> SER TYR
>> >> >>> > ILE TYR ILE LEU ILE THR ILE LEU LYS MET HID SER THR LYS GLY ARG
>> HIE
>> >> LYS
>> >> >>> ALA PHE
>> >> >>> > SER THR CYS THR SER HID LEU THR ALA VAL THR LEU PHE TYR GLY THR
>> ILE
>> >> THR
>> >> >>> PHE ILE
>> >> >>> > TYR VAL MET PRO LYS SER SER TYR SER THR ASP GLN ASN LYS VAL VAL
>> SER
>> >> VAL
>> >> >>> PHE TYR
>> >> >>> > THR VAL VAL ILE PRO MET LEU ASN PRO LEU ILE TYR SER LEU ARG ASN
>> LYS
>> >> GLU
>> >> >>> ILE LYS
>> >> >>> > GLY ALA LEU LYS ARG GLU LEU ARG ILE LYS ILE PHE SER
>> >> >>> >
>> >> >>> > how it would be possible to obtain some coordinates just from the
>> >> >>> > topology and trajectory using ambpdb? I'm working on cluster where
>> >> >>> > unfortunately there is no any visualization software.
>> >> >>> >
>> >> >>> > J.
>> >> >>> >
>> >> >>> > 2015-04-02 17:38 GMT+02:00 Kenneth Huang <
>> >> kennethneltharion.gmail.com <javascript:;> <javascript:;>>:
>> >> >>> >> Okay, so the error here isn't with your topologies- those are
>> fine.
>> >> It's
>> >> >>> >> that your topologies and coordinate files aren't matching up for
>> >> some
>> >> >>> >> reason. Assuming that they're the ones that you ran MD with, my
>> best
>> >> >>> guess
>> >> >>> >> is that there's something leftover that you forgot to strip out
>> >> when you
>> >> >>> >> ran ante-MMPBSA?
>> >> >>> >>
>> >> >>> >> You can also try making a pdb file with ambpdb using the same
>> >> inputs, or
>> >> >>> >> loading your trajectory with the relative topology into VMD to
>> see
>> >> if it
>> >> >>> >> works. If both of them fail, then something is missing from your
>> >> >>> topologies
>> >> >>> >> that's still in your trajectory.
>> >> >>> >>
>> >> >>> >> Best,
>> >> >>> >>
>> >> >>> >> Kenneth
>> >> >>> >>
>> >> >>> >> On Thu, Apr 2, 2015 at 11:08 AM, James Starlight <
>> >> >>> jmsstarlight.gmail.com <javascript:;> <javascript:;>>
>> >> >>> >> wrote:
>> >> >>> >>
>> >> >>> >>> what I found on the bottom
>> >> >>> >>>
>> >> >>> >>>
>> >> >>> >>> FATAL: NATOM mismatch in coord and topology files
>> >> >>> >>>
>> >> >>> >>>
>> >> >>> >>> I've checked all topologies and it seems ok for me
>> >> >>> >>>
>> >> >>> >>> n atoms of complex.prmtop = n atoms of receptor.prmtop + n
>> atoms of
>> >> >>> >>> ligand.prmtop
>> >> >>> >>>
>> >> >>> >>> it;s strange because I don't see here where the coordinate file
>> >> (smth
>> >> >>> >>> like protein.inpcrd) are provided in the below mmgbsa input
>> >> >>> >>>
>> >> >>> >>> mpirun -np 16 MMPBSA.py.MPI -O -i mmgbsa.in -o
>> >> >>> >>> mmgbsa_nm_OR5P3_quinoline.dat -sp
>> >> >>> >>> /home/cmoon/argh/OR5P3_quinoline/protein.parm7 -cp
>> >> >>> >>>
>> >> >>> >>>
>> >> >>>
>> >>
>> /home/cmoon/argh/OR5P3_quinoline/decomposition_OR5P3_quinoline/complex.prmtop
>> >> >>> >>> -rp
>> >> >>> >>>
>> >> >>>
>> >>
>> /home/cmoon/argh/OR5P3_quinoline/decomposition_OR5P3_quinoline/receptor.prmtop
>> >> >>> >>> -y /home/cmoon/argh/OR5P3_quinoline/md3.nc -lp
>> >> >>> >>>
>> >> >>> >>>
>> >> >>>
>> >>
>> /home/cmoon/argh/OR5P3_quinoline/decomposition_OR5P3_quinoline/ligand.prmtop
>> >> >>> >>> > progress.log 2>&1
>> >> >>> >>>
>> >> >>> >>>
>> >> >>> >>>
>> >> >>> >>> 2015-04-02 16:50 GMT+02:00 Kenneth Huang <
>> >> kennethneltharion.gmail.com <javascript:;> <javascript:;>
>> >> >>> >:
>> >> >>> >>> > James,
>> >> >>> >>> >
>> >> >>> >>> > Generally speaking, you can always make the stripped
>> topologies
>> >> you
>> >> >>> need
>> >> >>> >>> in
>> >> >>> >>> > tleap, usually before you add ions and solvent, or you could
>> just
>> >> >>> reload
>> >> >>> >>> > your starting structure into tleap and save it without
>> >> solvent/ions.
>> >> >>> That
>> >> >>> >>> > said, ante-MMPBSA is good for when you don't have your
>> starting
>> >> >>> structure
>> >> >>> >>> > anymore, or don't want to backtrack, or don't know what masks
>> you
>> >> >>> want to
>> >> >>> >>> > use.
>> >> >>> >>> >
>> >> >>> >>> > As for your error, try checking the MMPBSA gb mdout file- it
>> >> should
>> >> >>> give
>> >> >>> >>> > you a more descriptive error message.
>> >> >>> >>> >
>> >> >>> >>> > Best,
>> >> >>> >>> >
>> >> >>> >>> > Kenneth
>> >> >>> >>> >
>> >> >>> >>> > On Thursday, April 2, 2015, James Starlight <
>> >> jmsstarlight.gmail.com <javascript:;> <javascript:;>
>> >> >>> >>> > <javascript:_e(%7B%7D,'cvml','jmsstarlight.gmail.com
>> <javascript:;>
>> >> <javascript:;>');>> wrote:
>> >> >>> >>> >
>> >> >>> >>> >> Dear Friends!
>> >> >>> >>> >>
>> >> >>> >>> >> I've faced with the problem during mmgbsa analysis of my
>> system:
>> >> >>> >>> >>
>> >> >>> >>> >>
>> >> >>> >>> >>
>> >> >>> >>> >> Firstly I have no problems with processing of initial
>> >> protein.parm7
>> >> >>> >>> >> obtained using
>> >> >>> >>> >>
>> >> >>> >>> >> ante-MMPBSA.py -p ${sim}/protein.parm7 -c
>> >> >>> >>> >> ${sim}/decomposition_${simulation}/complex.prmtop -r
>> >> >>> >>> >> ${sim}/decomposition_${simulation}/receptor.prmtop -l
>> >> >>> >>> >> ${sim}/decomposition_${simulation}/ligand.prmtop -s
>> >> >>> >>> >> :WAT:Cl-:NA+:K+:PPC -n :MOL
>> >> >>> >>> >>
>> >> >>> >>> >> Stripping :WAT:Cl-:NA+:K+:PPC (solvent) from original
>> topology,
>> >> >>> output
>> >> >>> >>> >> is
>> >> >>> >>> >>
>> >> >>> >>>
>> >> >>>
>> >>
>> /home/cmoon/total_decomp/OR5P3_androstenone/decomposition_OR5P3_androstenone/complex.prmtop
>> >> >>> >>> >> Done stripping solvent!
>> >> >>> >>> >>
>> >> >>> >>> >> Creating receptor topology file by stripping :MOL from
>> >> >>> >>> >>
>> >> >>> >>> >>
>> >> >>> >>>
>> >> >>>
>> >>
>> /home/cmoon/total_decomp/OR5P3_androstenone/decomposition_OR5P3_androstenone/complex.prmtop
>> >> >>> >>> >> Done creating receptor topology file!
>> >> >>> >>> >>
>> >> >>> >>> >> Creating ligand topology file by stripping !(:MOL) from
>> >> >>> >>> >>
>> >> >>> >>> >>
>> >> >>> >>>
>> >> >>>
>> >>
>> /home/cmoon/total_decomp/OR5P3_androstenone/decomposition_OR5P3_androstenone/complex.prmtop
>> >> >>> >>> >> Done creating ligand topology file!
>> >> >>> >>> >>
>> >> >>> >>> >>
>> >> >>> >>> >>
>> >> >>> >>> >> but then during decomposition using mmgbsa.py I've obtained
>> >> error at
>> >> >>> >>> >> the begining of calculations:
>> >> >>> >>> >> (here the input file consisted of paths because it was
>> produced
>> >> by
>> >> >>> my
>> >> >>> >>> >> bash script)
>> >> >>> >>> >> mpirun -np 16 MMPBSA.py.MPI -O -i mmgbsa.in -o
>> >> >>> >>> >> mmgbsa_nm_OR5P3_androstenone.dat -sp
>> >> >>> >>> >> /home/cmoon/total_decomp/OR5P3_androstenone/protein.parm7 -cp
>> >> >>> >>> >> complex.prmtop -rp receptor.prmtop -y
>> >> >>> >>> >> /home/cmoon/total_decomp/OR5P3_androstenone/md1.nc
>> >> >>> >>> >> /home/cmoon/total_decomp/OR5P3_androstenone/md2.nc
>> >> >>> >>> >> /home/cmoon/total_decomp/OR5P3_androstenone/md3.nc -lp
>> >> >>> ligand.prmtop >
>> >> >>> >>> >> progress.log 2>&1
>> >> >>> >>> >>
>> >> >>> >>> >> ::
>> >> >>> >>> >> Running calculations on normal system...
>> >> >>> >>> >>
>> >> >>> >>> >> Beginning GB calculations with
>> >> /home/cmoon/Prog/amber12/bin/sander
>> >> >>> >>> >> calculating complex contribution...
>> >> >>> >>> >> CalcError: /home/cmoon/Prog/amber12/bin/sander failed with
>> >> prmtop
>> >> >>> >>> >> complex.prmtop!
>> >> >>> >>> >> Error occured on rank 3.
>> >> >>> >>> >> Exiting. All files have been retained.
>> >> >>> >>> >> application called MPI_Abort(MPI_COMM_WORLD, 1) - process 3
>> >> >>> >>> >> APPLICATION TERMINATED WITH THE EXIT STRING: Hangup (signal
>> 1)
>> >> >>> >>> >>
>> >> >>> >>> >> I've checked all prmtop files used here but have not found
>> any
>> >> >>> errors
>> >> >>> >>> >> here (the atom numbers and its composition is correct in all
>> 3
>> >> >>> >>> >> topologies made by ante-mmbsa). Could someone suggest me some
>> >> >>> >>> >> resolution if the issue and alternative method to made
>> stripped
>> >> >>> >>> >> topologies avoiding ante-mmbsa?
>> >> >>> >>> >>
>> >> >>> >>> >> Thanks!!
>> >> >>> >>> >>
>> >> >>> >>> >> James
>> >> >>> >>> >>
>> >> >>> >>> >> _______________________________________________
>> >> >>> >>> >> AMBER mailing list
>> >> >>> >>> >> AMBER.ambermd.org <javascript:;> <javascript:;>
>> >> >>> >>> >> http://lists.ambermd.org/mailman/listinfo/amber
>> >> >>> >>> >>
>> >> >>> >>> >
>> >> >>> >>> >
>> >> >>> >>> > --
>> >> >>> >>> > Ask yourselves, all of you, what power would hell have if
>> those
>> >> >>> >>> imprisoned
>> >> >>> >>> > here could not dream of heaven?
>> >> >>> >>> > _______________________________________________
>> >> >>> >>> > AMBER mailing list
>> >> >>> >>> > AMBER.ambermd.org <javascript:;> <javascript:;>
>> >> >>> >>> > http://lists.ambermd.org/mailman/listinfo/amber
>> >> >>> >>>
>> >> >>> >>> _______________________________________________
>> >> >>> >>> AMBER mailing list
>> >> >>> >>> AMBER.ambermd.org <javascript:;> <javascript:;>
>> >> >>> >>> http://lists.ambermd.org/mailman/listinfo/amber
>> >> >>> >>>
>> >> >>> >>
>> >> >>> >>
>> >> >>> >>
>> >> >>> >> --
>> >> >>> >> Ask yourselves, all of you, what power would hell have if those
>> >> >>> imprisoned
>> >> >>> >> here could not dream of heaven?
>> >> >>> >> _______________________________________________
>> >> >>> >> AMBER mailing list
>> >> >>> >> AMBER.ambermd.org <javascript:;> <javascript:;>
>> >> >>> >> http://lists.ambermd.org/mailman/listinfo/amber
>> >> >>>
>> >> >>> _______________________________________________
>> >> >>> AMBER mailing list
>> >> >>> AMBER.ambermd.org <javascript:;> <javascript:;>
>> >> >>> http://lists.ambermd.org/mailman/listinfo/amber
>> >> >>>
>> >> >> _______________________________________________
>> >> >> AMBER mailing list
>> >> >> AMBER.ambermd.org <javascript:;> <javascript:;>
>> >> >> http://lists.ambermd.org/mailman/listinfo/amber
>> >>
>> >> _______________________________________________
>> >> AMBER mailing list
>> >> AMBER.ambermd.org <javascript:;> <javascript:;>
>> >> http://lists.ambermd.org/mailman/listinfo/amber
>> >>
>> >
>> >
>> > --
>> > Ask yourselves, all of you, what power would hell have if those
>> imprisoned
>> > here could not dream of heaven?
>> > _______________________________________________
>> > AMBER mailing list
>> > AMBER.ambermd.org <javascript:;>
>> > http://lists.ambermd.org/mailman/listinfo/amber
>>
>> _______________________________________________
>> AMBER mailing list
>> AMBER.ambermd.org <javascript:;>
>> http://lists.ambermd.org/mailman/listinfo/amber
>>
>
>
> --
> Ask yourselves, all of you, what power would hell have if those imprisoned
> here could not dream of heaven?
> _______________________________________________
> AMBER mailing list
> AMBER.ambermd.org
> http://lists.ambermd.org/mailman/listinfo/amber
_______________________________________________
AMBER mailing list
AMBER.ambermd.org
http://lists.ambermd.org/mailman/listinfo/amber
Received on Tue Apr 07 2015 - 05:30:04 PDT
