That's great to hear!
Though, I am a little confused as to what you mean about using the stripped
topologies/trajectories for ante-MMPBSA- did you mean that you stripped the
trajectory and topology with cpptraj, and then used the stripped topology
in ante-MMPBSA as the complex? Just wondering, both for future reference if
anyone else runs into the problem and to see what exactly is going wrong in
the topology creation step.
Best,
Kenneth
On Monday, April 6, 2015, James Starlight <jmsstarlight.gmail.com> wrote:
> solved solved ! )
> What I've done is
>
> 1) providing protein.inpcrd at the reference during cutting of my
> trajectories using above script.
>
> 2) stripping solvent manually using cpptraj providing to ante-mmpbsa
> stripped trajectories and topologies as the input to made complex
> (which will be the same as the input topology in this case) as well as
> for complex and ligand.
>
> After this mmbsa have been finished without any error.
>
> In any of case thank for the help!
>
> Regards,
>
> James
>
> 2015-04-06 16:23 GMT+02:00 Kenneth Huang <kennethneltharion.gmail.com
> <javascript:;>>:
> > Assuming that you've treated the trajectories all the same before or
> after
> > merging them, ie applying the same strip masks, etc, then there shouldn't
> > be a problem.
> >
> > I don't think MMPBSA should care too much about where your coordinates
> are,
> > though it never hurts to autoimage and center your trajectory to a
> > reference. This seems more to be there's something present in the
> > trajectory or topology from the error message.
> >
> > Best,
> >
> > Kenneth
> >
> > On Monday, April 6, 2015, James Starlight <jmsstarlight.gmail.com
> <javascript:;>> wrote:
> >
> >> one important suggestion regarding my issue:
> >>
> >> I've tried to make this type of mmgbsa for merged trajectory consisted
> >> of 3 trajectory (each of which has been calculated for same input
> >> prmtop file) using below script to cut it together:
> >>
> >> parm protein.parm7
> >> trajin md1.nc 100 last 1
> >> trajin md2.nc 100 last 1
> >> trajin md3.nc 100 last 1
> >> trajout merged_md1_2_3.nc netcdf
> >>
> >> should I provide here some reference also to superimpose all of those
> >> trajectories onto common reference? What also might be relevant?
> >>
> >> James
> >> ~
> >> ~
> >> ~
> >> ~
> >> ~
> >>
> >> 2015-04-06 15:12 GMT+02:00 James Starlight <jmsstarlight.gmail.com
> <javascript:;>
> >> <javascript:;>>:
> >> > no no it was exactly paramtop of the whole system loaded with full
> >> > trajectory as you can found from
> >> >
> >> > 0: OR5P3_plus-carvone.parm7, 44513 atoms, 9262 res, box: Orthogonal,
> >> > 8970 mol, 8856 solvent, 5901 frames
> >> > just try to switch to the ambertools-14 and let you know about success
> >> >
> >> >
> >> >
> >> > James
> >> >
> >> > 2015-04-03 17:22 GMT+02:00 Kenneth Huang <kennethneltharion.gmail.com
> <javascript:;>
> >> <javascript:;>>:
> >> >>>
> >> >>> how it would be possible to obtain some coordinates just from the
> >> >>> topology and trajectory using ambpdb? I'm working on cluster where
> >> >>> unfortunately there is no any visualization software.
> >> >>
> >> >>
> >> >> The general command for ambpdb is
> >> >>
> >> >> ambpdb -p topology.prmtop < restart.inpcrd > OutputName.pdb
> >> >>
> >> >> Usually I use it with the starting .inpcrd or .restrt files- I
> haven't
> >> >> tried it with a trajectory, but I assume that you can just save a
> single
> >> >> frame, and use that as your coordinate file for ambpdb.
> Alternatively,
> >> you
> >> >> can save a single frame and move it along with the topologies onto a
> >> local
> >> >> machine to try to visualize it there.
> >> >>
> >> >>
> >> >>> also I've load both prmtop and nc into the cpptraj and no mismatch
> has
> >> >>> been found
> >> >>
> >> >>
> >> >> I could be wrong, but it looks like you've selected the initial
> prmtop
> >> and
> >> >> your trajectory- try loading a stripped version of your trajectory
> >> (using
> >> >> the same masks that you chose in running ante-MMPBSA) and its
> >> corresponding
> >> >> complex prmtop. Since I assume you can load your initial
> >> prmtop/trajectory
> >> >> without issue, I'm thinking that the issue is somewhere in the
> >> >> complex.prmtop file.
> >> >>
> >> >> just noticed that on cluster I'm using amber-tools-13. Was some bug
> >> >>> within mmpbsa.py of amber-tools of this version?
> >> >>
> >> >>
> >> >> I'm not sure if AmberTools13 had any problems associated with this,
> so
> >> >> you'd have to wait for someone else to weigh in. That said, I'm
> usually
> >> in
> >> >> favor of using newer versions of AmberTools, ie AmberTools14, unless
> you
> >> >> have a specific reason not to.
> >> >>
> >> >> Best,
> >> >>
> >> >> Kenneth
> >> >>
> >> >> On Friday, April 3, 2015, James Starlight <jmsstarlight.gmail.com
> <javascript:;>
> >> <javascript:;>> wrote:
> >> >>
> >> >>> also I've load both prmtop and nc into the cpptraj and no mismatch
> has
> >> >>> been found
> >> >>>
> >> >>> CPPTRAJ: Trajectory Analysis. V13.24
> >> >>> ___ ___ ___ ___
> >> >>> | \/ | \/ | \/ |
> >> >>> _|_/\_|_/\_|_/\_|_
> >> >>> INPUT: Reading Input from file xz.in
> >> >>> [parm OR5P3_plus-carvone.parm7]
> >> >>> AmberParm Title: [default_name]
> >> >>> Radius Set: modified Bondi radii (mbondi)
> >> >>> [trajin OR5P3_plus-carvone.nc netcdf 100 last 1]
> >> >>> [OR5P3_plus-carvone.nc] contains 6000 frames.
> >> >>> [trajout md_5p3_coumarin_md1_2.dcd dcd first]
> >> >>>
> >> >>> PARAMETER FILES:
> >> >>> 0: OR5P3_plus-carvone.parm7, 44513 atoms, 9262 res, box:
> Orthogonal,
> >> >>> 8970 mol, 8856 solvent, 5901 frames
> >> >>>
> >> >>> INPUT TRAJECTORIES:
> >> >>> 0: [OR5P3_plus-carvone.nc] is a NetCDF AMBER trajectory, Parm
> >> >>> OR5P3_plus-carvone.parm7 (Orthogonal box) (reading 5901 of 6000)
> >> >>> Coordinate processing will occur on 5901 frames.
> >> >>>
> >> >>>
> >> >>> just noticed that on cluster I'm using amber-tools-13. Was some bug
> >> >>> within mmpbsa.py of amber-tools of this version?
> >> >>>
> >> >>> J
> >> >>>
> >> >>> 2015-04-03 13:53 GMT+02:00 James Starlight <jmsstarlight.gmail.com
> <javascript:;>
> >> <javascript:;>>:
> >> >>> > I've checked it- solvent has been stripped correctly! and yes I'm
> >> >>> > using the same prmtop which I've used as the input to produce
> >> >>> > trajectory
> >> >>> > residues from complex:
> >> >>> > %FORMAT(20a4)
> >> >>> > GLU ASP THR THR VAL CYS ALA ILE LEU PHE LEU VAL PHE LEU GLY ILE
> TYR
> >> VAL
> >> >>> VAL THR
> >> >>> > LEU MET GLY ASN ILE SER ILE ILE VAL LEU ILE ARG ARG SER HID HIP
> LEU
> >> HID
> >> >>> THR PRO
> >> >>> > MET TYR ILE PHE LEU CYS HID LEU ALA PHE VAL ASH ILE GLY TYR SER
> SER
> >> SER
> >> >>> VAL THR
> >> >>> > PRO VAL MET LEU MET SER PHE LEU ARG LYS GLH THR SER LEU PRO VAL
> ALA
> >> GLY
> >> >>> CYX VAL
> >> >>> > ALA GLN LEU CYS SER VAL VAL THR PHE GLY THR ALA GLH CYS PHE LEU
> LEU
> >> ALA
> >> >>> ALA MET
> >> >>> > ALA TYR ASP ARG TYR VAL ALA ILE CYS SER PRO LEU LEU TYR SER THR
> CYS
> >> MET
> >> >>> SER PRO
> >> >>> > GLY VAL CYS ILE ILE LEU VAL GLY MET SER TYR LEU GLY GLY CYS VAL
> ASN
> >> ALA
> >> >>> TRP THR
> >> >>> > PHE ILE GLY CYS LEU LEU ARG LEU SER PHE CYS GLY PRO ASN LYS VAL
> ASN
> >> HIP
> >> >>> PHE PHE
> >> >>> > CYX ASP TYR SER PRO LEU LEU LYS LEU ALA CYS SER HID ASP PHE THR
> PHE
> >> GLU
> >> >>> ILE ILE
> >> >>> > PRO ALA ILE SER SER GLY SER ILE ILE VAL ALA THR VAL CYS VAL ILE
> ALA
> >> ILE
> >> >>> SER TYR
> >> >>> > ILE TYR ILE LEU ILE THR ILE LEU LYS MET HID SER THR LYS GLY ARG
> HIE
> >> LYS
> >> >>> ALA PHE
> >> >>> > SER THR CYS THR SER HID LEU THR ALA VAL THR LEU PHE TYR GLY THR
> ILE
> >> THR
> >> >>> PHE ILE
> >> >>> > TYR VAL MET PRO LYS SER SER TYR SER THR ASP GLN ASN LYS VAL VAL
> SER
> >> VAL
> >> >>> PHE TYR
> >> >>> > THR VAL VAL ILE PRO MET LEU ASN PRO LEU ILE TYR SER LEU ARG ASN
> LYS
> >> GLU
> >> >>> ILE LYS
> >> >>> > GLY ALA LEU LYS ARG GLU LEU ARG ILE LYS ILE PHE SER MOL
> >> >>> >
> >> >>> > residues from receptor:
> >> >>> >
> >> >>> > %FORMAT(20a4)
> >> >>> > GLU ASP THR THR VAL CYS ALA ILE LEU PHE LEU VAL PHE LEU GLY ILE
> TYR
> >> VAL
> >> >>> VAL THR
> >> >>> > LEU MET GLY ASN ILE SER ILE ILE VAL LEU ILE ARG ARG SER HID HIP
> LEU
> >> HID
> >> >>> THR PRO
> >> >>> > MET TYR ILE PHE LEU CYS HID LEU ALA PHE VAL ASH ILE GLY TYR SER
> SER
> >> SER
> >> >>> VAL THR
> >> >>> > PRO VAL MET LEU MET SER PHE LEU ARG LYS GLH THR SER LEU PRO VAL
> ALA
> >> GLY
> >> >>> CYX VAL
> >> >>> > ALA GLN LEU CYS SER VAL VAL THR PHE GLY THR ALA GLH CYS PHE LEU
> LEU
> >> ALA
> >> >>> ALA MET
> >> >>> > ALA TYR ASP ARG TYR VAL ALA ILE CYS SER PRO LEU LEU TYR SER THR
> CYS
> >> MET
> >> >>> SER PRO
> >> >>> > GLY VAL CYS ILE ILE LEU VAL GLY MET SER TYR LEU GLY GLY CYS VAL
> ASN
> >> ALA
> >> >>> TRP THR
> >> >>> > PHE ILE GLY CYS LEU LEU ARG LEU SER PHE CYS GLY PRO ASN LYS VAL
> ASN
> >> HIP
> >> >>> PHE PHE
> >> >>> > CYX ASP TYR SER PRO LEU LEU LYS LEU ALA CYS SER HID ASP PHE THR
> PHE
> >> GLU
> >> >>> ILE ILE
> >> >>> > PRO ALA ILE SER SER GLY SER ILE ILE VAL ALA THR VAL CYS VAL ILE
> ALA
> >> ILE
> >> >>> SER TYR
> >> >>> > ILE TYR ILE LEU ILE THR ILE LEU LYS MET HID SER THR LYS GLY ARG
> HIE
> >> LYS
> >> >>> ALA PHE
> >> >>> > SER THR CYS THR SER HID LEU THR ALA VAL THR LEU PHE TYR GLY THR
> ILE
> >> THR
> >> >>> PHE ILE
> >> >>> > TYR VAL MET PRO LYS SER SER TYR SER THR ASP GLN ASN LYS VAL VAL
> SER
> >> VAL
> >> >>> PHE TYR
> >> >>> > THR VAL VAL ILE PRO MET LEU ASN PRO LEU ILE TYR SER LEU ARG ASN
> LYS
> >> GLU
> >> >>> ILE LYS
> >> >>> > GLY ALA LEU LYS ARG GLU LEU ARG ILE LYS ILE PHE SER
> >> >>> >
> >> >>> > how it would be possible to obtain some coordinates just from the
> >> >>> > topology and trajectory using ambpdb? I'm working on cluster where
> >> >>> > unfortunately there is no any visualization software.
> >> >>> >
> >> >>> > J.
> >> >>> >
> >> >>> > 2015-04-02 17:38 GMT+02:00 Kenneth Huang <
> >> kennethneltharion.gmail.com <javascript:;> <javascript:;>>:
> >> >>> >> Okay, so the error here isn't with your topologies- those are
> fine.
> >> It's
> >> >>> >> that your topologies and coordinate files aren't matching up for
> >> some
> >> >>> >> reason. Assuming that they're the ones that you ran MD with, my
> best
> >> >>> guess
> >> >>> >> is that there's something leftover that you forgot to strip out
> >> when you
> >> >>> >> ran ante-MMPBSA?
> >> >>> >>
> >> >>> >> You can also try making a pdb file with ambpdb using the same
> >> inputs, or
> >> >>> >> loading your trajectory with the relative topology into VMD to
> see
> >> if it
> >> >>> >> works. If both of them fail, then something is missing from your
> >> >>> topologies
> >> >>> >> that's still in your trajectory.
> >> >>> >>
> >> >>> >> Best,
> >> >>> >>
> >> >>> >> Kenneth
> >> >>> >>
> >> >>> >> On Thu, Apr 2, 2015 at 11:08 AM, James Starlight <
> >> >>> jmsstarlight.gmail.com <javascript:;> <javascript:;>>
> >> >>> >> wrote:
> >> >>> >>
> >> >>> >>> what I found on the bottom
> >> >>> >>>
> >> >>> >>>
> >> >>> >>> FATAL: NATOM mismatch in coord and topology files
> >> >>> >>>
> >> >>> >>>
> >> >>> >>> I've checked all topologies and it seems ok for me
> >> >>> >>>
> >> >>> >>> n atoms of complex.prmtop = n atoms of receptor.prmtop + n
> atoms of
> >> >>> >>> ligand.prmtop
> >> >>> >>>
> >> >>> >>> it;s strange because I don't see here where the coordinate file
> >> (smth
> >> >>> >>> like protein.inpcrd) are provided in the below mmgbsa input
> >> >>> >>>
> >> >>> >>> mpirun -np 16 MMPBSA.py.MPI -O -i mmgbsa.in -o
> >> >>> >>> mmgbsa_nm_OR5P3_quinoline.dat -sp
> >> >>> >>> /home/cmoon/argh/OR5P3_quinoline/protein.parm7 -cp
> >> >>> >>>
> >> >>> >>>
> >> >>>
> >>
> /home/cmoon/argh/OR5P3_quinoline/decomposition_OR5P3_quinoline/complex.prmtop
> >> >>> >>> -rp
> >> >>> >>>
> >> >>>
> >>
> /home/cmoon/argh/OR5P3_quinoline/decomposition_OR5P3_quinoline/receptor.prmtop
> >> >>> >>> -y /home/cmoon/argh/OR5P3_quinoline/md3.nc -lp
> >> >>> >>>
> >> >>> >>>
> >> >>>
> >>
> /home/cmoon/argh/OR5P3_quinoline/decomposition_OR5P3_quinoline/ligand.prmtop
> >> >>> >>> > progress.log 2>&1
> >> >>> >>>
> >> >>> >>>
> >> >>> >>>
> >> >>> >>> 2015-04-02 16:50 GMT+02:00 Kenneth Huang <
> >> kennethneltharion.gmail.com <javascript:;> <javascript:;>
> >> >>> >:
> >> >>> >>> > James,
> >> >>> >>> >
> >> >>> >>> > Generally speaking, you can always make the stripped
> topologies
> >> you
> >> >>> need
> >> >>> >>> in
> >> >>> >>> > tleap, usually before you add ions and solvent, or you could
> just
> >> >>> reload
> >> >>> >>> > your starting structure into tleap and save it without
> >> solvent/ions.
> >> >>> That
> >> >>> >>> > said, ante-MMPBSA is good for when you don't have your
> starting
> >> >>> structure
> >> >>> >>> > anymore, or don't want to backtrack, or don't know what masks
> you
> >> >>> want to
> >> >>> >>> > use.
> >> >>> >>> >
> >> >>> >>> > As for your error, try checking the MMPBSA gb mdout file- it
> >> should
> >> >>> give
> >> >>> >>> > you a more descriptive error message.
> >> >>> >>> >
> >> >>> >>> > Best,
> >> >>> >>> >
> >> >>> >>> > Kenneth
> >> >>> >>> >
> >> >>> >>> > On Thursday, April 2, 2015, James Starlight <
> >> jmsstarlight.gmail.com <javascript:;> <javascript:;>
> >> >>> >>> > <javascript:_e(%7B%7D,'cvml','jmsstarlight.gmail.com
> <javascript:;>
> >> <javascript:;>');>> wrote:
> >> >>> >>> >
> >> >>> >>> >> Dear Friends!
> >> >>> >>> >>
> >> >>> >>> >> I've faced with the problem during mmgbsa analysis of my
> system:
> >> >>> >>> >>
> >> >>> >>> >>
> >> >>> >>> >>
> >> >>> >>> >> Firstly I have no problems with processing of initial
> >> protein.parm7
> >> >>> >>> >> obtained using
> >> >>> >>> >>
> >> >>> >>> >> ante-MMPBSA.py -p ${sim}/protein.parm7 -c
> >> >>> >>> >> ${sim}/decomposition_${simulation}/complex.prmtop -r
> >> >>> >>> >> ${sim}/decomposition_${simulation}/receptor.prmtop -l
> >> >>> >>> >> ${sim}/decomposition_${simulation}/ligand.prmtop -s
> >> >>> >>> >> :WAT:Cl-:NA+:K+:PPC -n :MOL
> >> >>> >>> >>
> >> >>> >>> >> Stripping :WAT:Cl-:NA+:K+:PPC (solvent) from original
> topology,
> >> >>> output
> >> >>> >>> >> is
> >> >>> >>> >>
> >> >>> >>>
> >> >>>
> >>
> /home/cmoon/total_decomp/OR5P3_androstenone/decomposition_OR5P3_androstenone/complex.prmtop
> >> >>> >>> >> Done stripping solvent!
> >> >>> >>> >>
> >> >>> >>> >> Creating receptor topology file by stripping :MOL from
> >> >>> >>> >>
> >> >>> >>> >>
> >> >>> >>>
> >> >>>
> >>
> /home/cmoon/total_decomp/OR5P3_androstenone/decomposition_OR5P3_androstenone/complex.prmtop
> >> >>> >>> >> Done creating receptor topology file!
> >> >>> >>> >>
> >> >>> >>> >> Creating ligand topology file by stripping !(:MOL) from
> >> >>> >>> >>
> >> >>> >>> >>
> >> >>> >>>
> >> >>>
> >>
> /home/cmoon/total_decomp/OR5P3_androstenone/decomposition_OR5P3_androstenone/complex.prmtop
> >> >>> >>> >> Done creating ligand topology file!
> >> >>> >>> >>
> >> >>> >>> >>
> >> >>> >>> >>
> >> >>> >>> >> but then during decomposition using mmgbsa.py I've obtained
> >> error at
> >> >>> >>> >> the begining of calculations:
> >> >>> >>> >> (here the input file consisted of paths because it was
> produced
> >> by
> >> >>> my
> >> >>> >>> >> bash script)
> >> >>> >>> >> mpirun -np 16 MMPBSA.py.MPI -O -i mmgbsa.in -o
> >> >>> >>> >> mmgbsa_nm_OR5P3_androstenone.dat -sp
> >> >>> >>> >> /home/cmoon/total_decomp/OR5P3_androstenone/protein.parm7 -cp
> >> >>> >>> >> complex.prmtop -rp receptor.prmtop -y
> >> >>> >>> >> /home/cmoon/total_decomp/OR5P3_androstenone/md1.nc
> >> >>> >>> >> /home/cmoon/total_decomp/OR5P3_androstenone/md2.nc
> >> >>> >>> >> /home/cmoon/total_decomp/OR5P3_androstenone/md3.nc -lp
> >> >>> ligand.prmtop >
> >> >>> >>> >> progress.log 2>&1
> >> >>> >>> >>
> >> >>> >>> >> ::
> >> >>> >>> >> Running calculations on normal system...
> >> >>> >>> >>
> >> >>> >>> >> Beginning GB calculations with
> >> /home/cmoon/Prog/amber12/bin/sander
> >> >>> >>> >> calculating complex contribution...
> >> >>> >>> >> CalcError: /home/cmoon/Prog/amber12/bin/sander failed with
> >> prmtop
> >> >>> >>> >> complex.prmtop!
> >> >>> >>> >> Error occured on rank 3.
> >> >>> >>> >> Exiting. All files have been retained.
> >> >>> >>> >> application called MPI_Abort(MPI_COMM_WORLD, 1) - process 3
> >> >>> >>> >> APPLICATION TERMINATED WITH THE EXIT STRING: Hangup (signal
> 1)
> >> >>> >>> >>
> >> >>> >>> >> I've checked all prmtop files used here but have not found
> any
> >> >>> errors
> >> >>> >>> >> here (the atom numbers and its composition is correct in all
> 3
> >> >>> >>> >> topologies made by ante-mmbsa). Could someone suggest me some
> >> >>> >>> >> resolution if the issue and alternative method to made
> stripped
> >> >>> >>> >> topologies avoiding ante-mmbsa?
> >> >>> >>> >>
> >> >>> >>> >> Thanks!!
> >> >>> >>> >>
> >> >>> >>> >> James
> >> >>> >>> >>
> >> >>> >>> >> _______________________________________________
> >> >>> >>> >> AMBER mailing list
> >> >>> >>> >> AMBER.ambermd.org <javascript:;> <javascript:;>
> >> >>> >>> >> http://lists.ambermd.org/mailman/listinfo/amber
> >> >>> >>> >>
> >> >>> >>> >
> >> >>> >>> >
> >> >>> >>> > --
> >> >>> >>> > Ask yourselves, all of you, what power would hell have if
> those
> >> >>> >>> imprisoned
> >> >>> >>> > here could not dream of heaven?
> >> >>> >>> > _______________________________________________
> >> >>> >>> > AMBER mailing list
> >> >>> >>> > AMBER.ambermd.org <javascript:;> <javascript:;>
> >> >>> >>> > http://lists.ambermd.org/mailman/listinfo/amber
> >> >>> >>>
> >> >>> >>> _______________________________________________
> >> >>> >>> AMBER mailing list
> >> >>> >>> AMBER.ambermd.org <javascript:;> <javascript:;>
> >> >>> >>> http://lists.ambermd.org/mailman/listinfo/amber
> >> >>> >>>
> >> >>> >>
> >> >>> >>
> >> >>> >>
> >> >>> >> --
> >> >>> >> Ask yourselves, all of you, what power would hell have if those
> >> >>> imprisoned
> >> >>> >> here could not dream of heaven?
> >> >>> >> _______________________________________________
> >> >>> >> AMBER mailing list
> >> >>> >> AMBER.ambermd.org <javascript:;> <javascript:;>
> >> >>> >> http://lists.ambermd.org/mailman/listinfo/amber
> >> >>>
> >> >>> _______________________________________________
> >> >>> AMBER mailing list
> >> >>> AMBER.ambermd.org <javascript:;> <javascript:;>
> >> >>> http://lists.ambermd.org/mailman/listinfo/amber
> >> >>>
> >> >> _______________________________________________
> >> >> AMBER mailing list
> >> >> AMBER.ambermd.org <javascript:;> <javascript:;>
> >> >> http://lists.ambermd.org/mailman/listinfo/amber
> >>
> >> _______________________________________________
> >> AMBER mailing list
> >> AMBER.ambermd.org <javascript:;> <javascript:;>
> >> http://lists.ambermd.org/mailman/listinfo/amber
> >>
> >
> >
> > --
> > Ask yourselves, all of you, what power would hell have if those
> imprisoned
> > here could not dream of heaven?
> > _______________________________________________
> > AMBER mailing list
> > AMBER.ambermd.org <javascript:;>
> > http://lists.ambermd.org/mailman/listinfo/amber
>
> _______________________________________________
> AMBER mailing list
> AMBER.ambermd.org <javascript:;>
> http://lists.ambermd.org/mailman/listinfo/amber
>
--
Ask yourselves, all of you, what power would hell have if those imprisoned
here could not dream of heaven?
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Received on Mon Apr 06 2015 - 08:30:02 PDT
