Dear Sir/Madam,
I have done a PMF for a protein-drug dissociation process.
Large conformation change in the protein was observed. It opens up the space for the drug to dissociate from the binding site. I tends to interprete that as a conformation change during drug dissociation process.
However, could the large conformational change be only artifacts of fast pulling, because the timescales required for a partial protein unfolding is not consistent with experimental dissociation rates?
Could you mind to comments on how to interprete the conformation change in PMF of drug-dissociation?
Best regards & thanks,
Catherine
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Received on Thu Apr 07 2011 - 09:30:03 PDT
