> hello amber users,
> I tried to load a double stranded tRNA sequence having a
> minor RNA base INOSINE ( the sequence had been taken from pdb 1XNQ)
> in xleap. I used leaprc.rna.ff02 force field to start xleap, but
> unfortunately xleap could not recognise the INOSINE residue. I even
> loaded the all_modrna.ff08.lib, hoping that the situation will
> improve, INOSINE still remained unrecognised by xleap.
>
> When I tried loading another double stranded RNA sequence
> having all standard residues ( G and C), I could easily generate the
> prmtop and inpcrd files.
>
> Am I choosing a wrong force field for the INOSINE
> containing sequence?
>
> Kindly help me out.
>
> with regards, thanking in advance.
>
> Gunajyoti
> Neh university
