[AMBER] Question regarding fixing cispeptides in modelled loops and protonation states

From: Jenny 148 via AMBER <amber.ambermd.org>
Date: Thu, 22 May 2025 12:40:37 -0400

Hi everyone,

I am working on a multiprotein system. 2 of the subunits have multiple
missing regions. Since the structural template we use is unpublished we
want to refrain from using any webservers. I used the sequence info alone
to model the 2 subunits using alphafold3, then aligned it to the template
and pasted the sequence of the missing region alone. This however resulted
in some clashes and some cispeptide (non proline). I have 2 queries:

1. Do we have any tool in AMBER which might help us fix the cispeptide
problem?

2. How to predict the protonation states? I am aware of the H++ server. But
I wonder if there is someway without uploading our unpublished structure
into the server.

3. Is there any discrepancy in the method I am using to fill the missing
residues?

This is the first time I am working with a multiprotein system with really
large number of missing region which likely are not just loops.

Thank you for any insights you can provide.


-Jenny
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Received on Thu May 22 2025 - 10:00:02 PDT
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