[AMBER] How to patch phosphate at the 3'-end

From: Indrajit Deb <biky2004indra.gmail.com>
Date: Tue, 30 Aug 2011 17:25:17 +0530

Dear AMBER users,
I want to prepare single nucleotide. I mean phosphate at the C5'-end and
base at C1'. In the all_nuc94.in file RU5, RU3 etc are given, but the
problem is that in case of RU3 how to add OH group with the PO2 group at the
5'-end to close the chain and make it a nucleotide. In case of RU5 how to
add H at the C3'-end to close the chain and make it nucleoside.

I am waiting for helpful suggestions.

Thanks in advance

----indra


---------------------------------------------------------------------
Indrajit Deb
Dept. of Biophysics, Molecular biology and Bioinformatics
University of Calcutta
92 APC Road
Kolkata - 700 009. India.
Ph. +919239202278
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Received on Tue Aug 30 2011 - 05:00:03 PDT
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