NaCl concentration

From: Peter Gannett <>
Date: Tue 30 Apr 2002 15:34:38 -0400

Amber users:

I would like to run a DNA at 1 M NaCl and I am trying to decide how many Na+ and Cl- to add. I have taken the seemingly simple minded approach - I want 1 M NaCl, water is roughly 55.5 M, and my system has 2100 waters. Since the ratio of NaCl/water = 1/55.5, this gives 38 NaCl. But, since 10 Na+ are needed for the DNA phosphates, I've added 28 NaCl.

Is this a reasonable approach?


Received on Tue Apr 30 2002 - 12:34:38 PDT
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