I have recently mutated a 3' terminal base on a 14 mer DNA duplex. Upon
saving the amber parameter files I get the warning
"Residues lacking connect0/connedt1 - these don't have chain types marked"
>From other errors I received in getting the prep file ready, it seems that
this error comes from the 5' base (DG5) of the complement strand. Because
the mutated base (it actually has a dye attached by phosphate linker on the
terminal OH) no longer has a terminal OH group, I was wondering if the
complement is looking for it? Any suggestions?
Thanks,
Troy
Received on Wed Mar 28 2001 - 10:48:50 PST
