Checking cpin file and/or cein file. We are going to find DELTAGREF for a pH titratable residue without using Replica Exchange. The solvent pH value is 9.600 and was loaded from the mdin file (cp_ph.in). The temperature is 300.00 K and was loaded from the mdin file (cp_ph.in). According to the mdin file (cp_ph.in), the value of ntcnstph is 500. According to the mdin file (cp_ph.in), the value of nstlim is 10000000. The range of values for DELTAGREF is 0.000000 to 500.000000 kcal/mol (given on the argument -dgrefrange) AMBER execution #1: running 5000 MD steps for DELTAGREF = 0.000000 kcal/mol The fraction of protonated species is 0.00% for the Residue 'TYR 2' AMBER execution #2: running 5000 MD steps for DELTAGREF = 500.000000 kcal/mol