Is this helix (A)nti-parallel, (P)arallel, or (S)ingle stranded? A Anti-parallel double helix Which strand should be considered the primary strand (1-2)? 1 Which strand should be considered the complementary strand (1-2)? 2 What is the primary strand starting residue number (1-4)? 1 What is the primary strand ending residue number (1-4)? 4 What is the complementary strand starting residue number { paired with 1:1 } (1-4)? 4 What is the complementary strand ending residue number { paired with 1:4 } (1-4)? 1 Do you wish to use the default Pivot Point [ 0, 1.808, 0 ]? (y/n) y Does this helix have any non-Watson-Crick base pairs? (y/n) n *** Basepair Analysis : Strands 1 and 2. *** Basepair Buckle Prop Opening Angle Shear Stretch Stagger Dist 1: 1 CYT - 2: 4 GUA 0.01 -0.07 -3.74 3.74 -0.21 -0.03 0.01 0.21 1: 2 GUA - 2: 3 CYT -0.01 -0.07 -3.74 3.74 0.21 -0.03 0.01 0.21 1: 3 THY - 2: 2 ADE 0.16 0.49 -8.21 8.23 -0.16 0.09 -0.03 0.18 1: 4 ADE - 2: 1 THY -0.16 0.49 -8.21 8.23 0.16 0.09 -0.03 0.18 Mean 0.00 0.21 -5.98 5.99 -0.00 0.03 -0.01 0.20 Standard Deviation 0.13 0.32 2.58 2.59 0.21 0.07 0.02 0.02 *** Local Basepair Analysis : Strands 1 and 2. *** Top Basepair / Bottom Basepair Tilt Roll Twist Angle Shift Slide Rise Dist 1: 1 - 2: 4 / 1: 2 - 2: 3 :: 0.11 -3.22 38.00 38.13 0.01 -0.13 3.38 3.38 1: 2 - 2: 3 / 1: 3 - 2: 2 :: 0.01 -3.12 31.77 31.92 -0.07 -0.33 3.37 3.39 1: 3 - 2: 2 / 1: 4 - 2: 1 :: 0.02 -3.15 37.43 37.56 0.01 -0.23 3.37 3.38 Mean 0.04 -3.16 35.73 35.87 -0.02 -0.23 3.37 3.38 Standard Deviation 0.06 0.05 3.44 3.43 0.05 0.10 0.01 0.01 *** Local Helical Analysis : Strands 1 and 2. *** Top Basepair / Bottom Basepair Inclination Tip HTwist DX DY DZ 1: 1 - 2: 4 / 1: 2 - 2: 3 -4.85 -0.16 38.13 -0.03 -0.09 3.38 1: 2 - 2: 3 / 1: 3 - 2: 2 -5.61 -0.01 31.92 0.04 -0.01 3.39 1: 3 - 2: 2 / 1: 4 - 2: 1 -4.81 -0.03 37.56 -0.02 -0.03 3.38 Mean -5.09 -0.07 35.87 -0.00 -0.05 3.38 Standard Deviation 0.45 0.08 3.43 0.04 0.04 0.01 *** Sugar Pucker Analysis : Strand number 1. *** Res Type Alpha Beta Gamma Delta Epsilon Zeta Chi Pseudorotation 1: 1 CYT - 70.92 30.89 156.53 149.08 -124.57 -98.41 192.31 1: 2 GUA 5.04 -180.00 30.89 156.53 151.11 -120.60 -98.50 192.31 1: 3 THY -6.19 -180.00 30.89 156.53 157.39 -122.74 -100.92 192.31 1: 4 ADE -8.12 -180.00 30.89 156.53 - - -101.89 192.31 Mean -3.09 -117.27 30.89 156.53 152.53 -122.64 -99.93 192.31 Std Dev 4.18 125.46 0.00 0.00 4.33 1.99 1.75 0.00 *** Sugar Pucker Analysis : Strand number 2. *** Res Type Alpha Beta Gamma Delta Epsilon Zeta Chi Pseudorotation 2: 4 GUA 5.04 -180.00 30.89 156.53 - - -98.50 192.31 2: 3 CYT 6.98 -180.00 30.89 156.53 149.12 -124.58 -98.41 192.31 2: 2 ADE -8.46 -180.00 30.89 156.53 154.99 -128.71 -101.89 192.31 2: 1 THY - 33.54 30.89 156.53 157.04 -122.49 -100.92 192.31 Mean 1.19 -126.61 30.89 156.53 153.72 -125.26 -99.93 192.31 Std Dev 7.96 106.77 0.00 0.00 4.11 3.16 1.75 0.00