Hi,
You should be able to bond the Zn right into its coordination sphere. MCPB would help set up all the files to deal with this. BTW I think a CCCC-Zn finger was set up in this paper: http://pubs.acs.org/doi/abs/10.1021/ct1002626. Good luck. Kennie
On Jan 11, 2014, at 7:03 AM, Tong <tongzhu9110.gmail.com> wrote:
> Hi, You can use NMR restraint to maintain the structure...
>
>
> On 01/11/2014 06:47 PM, Yip Yew Mun wrote:
>> Hi,
>>
>> I'm currently planning to simulate a zinc finger where 4 cysteine residues are coordinated to a zinc ion. I changed the CYS to CYM. I also created a non-standard residue for the zinc ion. I wish to ask if I need to explicitly use the command "bond" to bond the SG atom of my CYM residues to the zinc ion.?
>>
>> Thanks.
>>
>> Sent from my iPhone
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Received on Sat Jan 11 2014 - 05:00:02 PST
