Dear users,
It is usually suggested that while preparing a nucleic acid system for
simulation, 5' terminal phosphate group must be removed; or any terminal
charged phosphate group should be removed...
Can you please explain why it should be like that??
If i am extracting a small RNA helix from a large molecule e.g. ribosomal
RNA, do i have to still remove those flanking phosphate groups?
Asmita
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Received on Sun Dec 16 2012 - 22:30:05 PST
