AMBER: single stranded DNA

From: Taufik Al-Sarraj <taufik.alsarraj.utoronto.ca>
Date: Tue, 06 May 2008 17:37:41 -0500

I apologize for the simplicity of this question,

I would like to create a single stranded DNA using nucgen, i used the
following (AMBER 9)

nuc.in
' NUC 1
D
A5 T T T T G T C T G A A A C C C T G T3

END
$ABDNA'

then
nucgen -O -i nuc.in -o nuc.out -d .../nucgen.dat -p nuc.pdb

I get a pdb file with the first 16 residues, even though i have 19. i
also get a double stranded DNA with 8 residues on each side.

should i take away the 1 after NUC? or should i split the residues into
two lines?

Thank you for your help,
Taufik






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Received on Wed May 07 2008 - 06:07:49 PDT
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